Diindolylmethane DIM supplement health benefit 100 mg pills, side effects and safety, for estrogen dominance? research study results and review
October 2 2016 by
Ray Sahelian, M.D.

DIM is an indole phytochemical that is a natural metabolic of compounds found in cruciferous vegetables such as broccoli, Brussels sprouts and cabbage. When an indole 3 carbinol supplement is ingested, it converts in the gut into diindolylmethane.

Source Naturals - buy DIM Diindolylmethane supplement 100 mg per pill, 120 Tablets

Buy DIM diindolylmethane

Supplement Facts
Serving Size: 1 Tablet
  Amount Per Serving %DV
Vitamin E (as d-alpha tocopheryl succinate) 50 IU 167%
Diindolylmethane (DIM) 100 mg
Lecithin (from soybeans) 100 mg
Black Pepper Fruit Extract (BioPerine) 3 mg
†Daily Value not established.

In animal and in-vitro studies.  DIM has been shown to lead to the preferential formation of estrogen, and cervical tissues.  This unique property sets DIM apart from other plant nutrients.  Source Naturals DIM is combined with phosphatidyl choline, vitamin E and bioperine for enhanced absorption.

Buy DIM supplement Diindolylmethane supplement tablets or see a list of high quality supplements
DIM Supplement Facts
Vitamin E (as D-alpha tocopheryl)
Diindolylmethane (DIM)
Phosphatidyl Choline
Pepper Fruit Extract (Bioperine)

Suggested Use: 1 to 2 diindolymethane tablets per day with meals, or as recommended. by your health care professional.

Additional nutrients and supplements that may play a role in cancer prevention or treatment
is a mushroom extract that has been tested as an anti-cancer agent.
Calcium D Glucarate is a natural supplement.
Indole3Carbinol I3C is available as a supplement.
   For a full list of natural supplements, herbs, nutritional substances and dietary factors that influence tumor prevention or treatment, see cancer.


Breast, ovarian cancer prevention
Researchers at the University of California, Los Angeles, found in lab studies that diindolymethane, a compound resulting from digestion of cruciferous vegetables, and genistein, an isoflavone in soy, reduce the production of two proteins needed for breast and ovarian cancer to spread. The UCLA team plans to evaluate the theory in mice.Cancer cell membranes have high levels of a surface receptor known as CXCR4, while the organs to which the cancers spread secrete high levels of CXCL12, a ligand that binds to that particular receptor. This attraction stimulates the invasive properties of cancer cells and acts like a homing device, drawing the cancer cells to organs like the liver or brain. When cancer cells were treated with either DIM or genistein, movement toward CXCL12 was significantly reduced compared to untreated cells.

Pilot study: effect of 3,3'- diindolylmethane supplements on urinary hormone metabolites in postmenopausal women with a history of early-stage breast cancer.
Nutr Cancer. 2004. Department of Molecular and Cell Biology, University of California, Berkeley, USA.
Dietary indoles, present in Brassica plants such as cabbage, broccoli, and Brussels sprouts, have been shown to provide potential protection against hormone-dependent cancers. 3,3'-Diindolylmethane is under study as one of the main protective indole metabolites. Postmenopausal women aged 50-70 yr from Marin County, California, with a history of early-stage breast cancer, were screened for interest and eligibility in this pilot study on the effect of absorbable DIM (BioResponse- diindolylmethane) supplements on urinary hormone metabolites. The treatment group received daily DIM (108 mg /day) supplements for 30 days, and the control group received a placebo capsule. Urinary metabolite analysis included 2-hydroxyestrone (2-OHE1), 16-alpha hydroxyestrone (16alpha-OHE1), diindolylmethane, estrone (El), estradiol(E2), estriol (E3), 6beta-hydroxycortisol (6beta-OHC), and cortisol in the first morning urine sample before intervention and 31 days after intervention. DIM-treated subjects showed a significant increase in levels of2-OHE1, diindolylmethane, and cortisol, and a nonsignificant increase in the 2-OHE1/16alpha-OHE1 ratio from 1.4 to 2.1. In this pilot study, DIM increased the 2-hydroxylation of estrogen urinary metabolites.

Combining diindolylmethane and paclitaxel for breast cancer
3,3'-diindolylmethane and paclitaxel act synergistically to promote apoptosis in HER2/Neu human breast cancer cells.
J Surg Res. 2006. Department of Surgery, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
About 25 to 30% of invasive breast tumors overexpress the HER2 / neu oncogene. These tumors are aggressive and become resistant to chemotherapeutic drugs. 3'3'-diindolylmethane (DIM), the active metabolite of indole-3-carbinol, a naturally occurring compound found in cruciferous vegetables, has been found to have anti-cancer properties in both humans and animals. DIM has been shown to induce cell cycle arrest and apoptosis in animal breast cancer models. Diindolylmethane in combination with paclitaxel synergistically inhibits growth of Her2 / neu human breast cancer cells through G2M phase cell-cycle arrest and induction of apoptosis / necrosis. The Her2 / neu receptor and its downstream signaling protein ERK1/2 appear to be involved in DIM's affect on cell growth and differentiation, whereas apoptosis appears to be mediated through the mitochondrial pathway (Bcl-2/PARP). It appears diindolylmethane, a naturally occurring, nontoxic compound, may be a beneficial addition to a traditional (taxane-based) chemotherapy regimen.

PLoS One. 2012. 3,3'-Diindolylmethane induces G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia cells. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, United States of America.

Nasal cancer
Mol Med Rep. 2015. Pro-apoptotic and anti-proliferative effects of 3,3'-diindolylmethane in nasopharyngeal carcinoma cells via downregulation of telomerase activity.

Prostate cancer
Diindolymethane appears to have antiproliferative and proapoptotic effects in prostate cancer cells. DIM -induced cell proliferation inhibition and apoptosis induction are partly mediated through the down-regulation of androgen receptors and NF-kappaB signaling.

3,3'-Diindolylmethane induces a G(1) arrest in human prostate cancer cells irrespective of androgen receptor and p53 status.
Biochem Pharmacol. 2009. Department of Nutritional Sciences and Toxicology, University of California, Berkeley, CA, USA.
In this study we characterized the effect of DIM on cell cycle regulation in both androgen-dependent LNCaP and androgen receptor negative p53 mutant DU145 human prostate cancer cells. DIM had an anti-proliferative effect on both LNCaP and DU145 cells, as it significantly inhibited [3H]-thymidine incorporation. Our results indicate that DIM is able to stop the cell cycle progression of human prostate cancer cells regardless of their androgen-dependence and p53 status, by differentially modulating cell cycle regulatory pathways. The Sp1 and p38 MAPK pathways mediate the DIM cell cycle regulatory effect in DU145 cells.

Role in cancer research and trials
Cancer Epidemiol Biomarkers Prev. 2014 Feb. Urinary 3,3'-diindolylmethane: a biomarker of glucobrassicin exposure and indole-3-carbinol uptake in humans. Brassica vegetable consumption may confer a protective effect against cancer, possibly attributable to their glucosinolates. Glucobrassicin is a predominant glucosinolate and is the precursor of indole-3-carbinol (I3C), a compound with anticancer effects. DIM is a measure of I3C uptake from Brassica vegetables, a finding that can be utilized in prospective epidemiologic and chemoprevention studies.

Is DIM diindolylmethane product for women only? Or, can it be used by men to help raise their testosterone?
   Not enough human research has been done with DIM diindolylmethane to determine which clinical conditions this supplement is useful for and how it effects hormone levels.

I would like to buy a stable form of DIM. Is the Bioresponse Dim supplement in some products the only DIM supplement that should be considered by consumers wishing to use a safe and effective form?
    We are not aware of any studies that have examined the different forms of DIM diindolylmethane products on the market to determine which are the most effective. As far as we know, Source Naturals DIM product is of high quality.

What would be the benefit for a woman that has had a complete hysterectomy (20 years ago) to take DIM. Also is it useful for men that take testosterone?
    There is not enough research on these topics to have a good answer at this time.

I am a chiropractor in San Diego, CA. Is this product pharmaceutical grade?
   The DIM product is made by Source Naturals and you can visit their website to ask them directly.

I tried several times to take diindolylmethane do address estrogen dominance but was getting really bad headaches and had to stop. Any ideas as to why I'm getting headaches and is there any way to overcome this side effect?
    It is difficult to know the reason, but you may consider taking a quarter or half a tablet with food.

Dim Source Naturals may have certain estrogen metabolizing effects. Does Passion Rx have any specific herbs that are known to have similar effects? If not, what is the thought about taking Passion Rx at the same time as Dim Source Naturals.
    We have not tested the combination. It is best to first try each supplement separately for a week or so before considering combining them. The sexual enhancing formula is not likely to have a major effect on estrogen metabolism.