Amyloglucosidase
February 1 2017
This is an enzyme of microbial origin that breaks glucoside bonds in starch and dextrins to form glucose; used in the manufacturing of glucose and for converting carbohydrates to fermentable sugars (as in beer-brewing). Also known as glucoamylase. For information on digestive enzymes.
On-site production of crude glucoamylase for kitchen
waste hydrolysis
Kitchen waste from dining rooms accounts for a considerable proportion of
municipal solid garbage, and economical recycle ways are needed to be developed.
This study investigated amyloglucosidase production from kitchen waste and the
feasibility of kitchen waste hydrolysis by the crude enzymes produced. The key
problems of high water content and poor porosity in kitchen waste for amyloglucosidase
production under solid-state fermentation could be solved readily by the
addition of corn stover or paddy husk. As a support medium, corn stover was
better than paddy husk. Smashed kitchen waste (sKW) mixed with corn stover in
the ratio of 3.75 : 1 (dry basis) produced 1838 U g(-1) of amyloglucosidase by
Aspergillus niger UV-60 within 96 h. The enzyme productivity from kitchen waste
was over two-fold higher than that from wheat bran with additional nutrients.
Without any recovery treatment, the produced amyloglucosidase could be used
directly to hydrolyse sKW slurry. Waste Manag Res. 2010. National
Engineering Laboratory for Biomass Power Generation Equipment, School of
Renewable Energy, North China Electric Power University, Beijing, China.